How to grow pseudomonas aeruginosa in lab?

Growing Pseudomonas aeruginosa in laboratory settings requires specific conditions and safety precautions. As someone who's worked with this organism in research:

P. aeruginosa is an opportunistic pathogen - always use BSL-2 precautions with proper personal protective equipment. It grows well on standard media like nutrient agar, tryptic soy agar, or specialized media like Pseudomonas isolation agar.

Optimal growth temperature is 37°C (human body temperature). It's aerobic so doesn't require special atmosphere. Incubate 18-24 hours for visible colonies, which often have characteristic grape-like odor and greenish coloration due to pyocyanin production.

For liquid culture, use nutrient broth or tryptic soy broth with shaking for aeration. It grows rapidly - can reach high cell density in 8-12 hours under ideal conditions.

ATCC's Pseudomonas aeruginosa guide provides specific culturing protocols. Always follow institutional safety protocols when working with pathogens!