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To measure VVMs, place the VVMs with the release liner still attached to white card stock, then measure the Optical Density (OD) of the active indicator (I) and reference ring (R) using an X-Rite 500 series spectrodensitometer or equivalent. Measure the reference ring by taking one measurement from the bottom of the ring and a second measurement from another point 90° away. Measure the active indicator by taking two measurements from different points within the active square. To determine the optical density difference, also called R-I, subtract the average I value from the average R-value.

Prior to being applied to vaccines, VVMs must be stored at temperatures < -24°C and kept away from light and other sources of radiation. Once applied to vaccines, storage conditions are based on the vaccine’s storage requirements.

VVMs help to expand the reach of immunization programs to remote populations and to minimize the risk of people receiving potentially ineffective, heat-damaged vaccines along with increasing the efficiency of immunization programs worldwide.

The VVM is a critical component of vaccine manufacturers' ability to supply life-saving vaccines in support of global vaccination programs. Through a simple, predictable, reliable, and visual color change, health workers know at a glance whether a vaccine has been exposed to potentially damaging heat. Irreversible time-temperature indicators can play a vital role in the success of vaccination programs worldwide.

Here's how VVMs are used in different settings:

1. Vaccine storage: VVMs can be affixed to vaccine vials during storage to monitor the temperature of the vaccine. When the vaccine is removed from storage, the healthcare worker can check the VVM to ensure that the vaccine has not been exposed to temperatures outside of the recommended range.

2. Vaccine transport: VVMs can be used during vaccine transport to monitor the temperature of the vaccines. The VVMs can provide an indication of whether the vaccines have been exposed to excessive heat or cold during transport, enabling healthcare workers to identify potential breaches in temperature control.

3. Vaccine administration: VVMs can be checked by healthcare workers before vaccine administration to ensure that the vaccine is still potent. If the VVM shows that the vaccine has been compromised due to temperature fluctuations, the healthcare worker can discard the vaccine and use an alternative one to ensure that the patient receives a potent vaccine.

In day-to-day vaccine management, VVMs provide critical information about the temperature history of vaccines, enabling healthcare workers to ensure that vaccines are properly maintained and effective. By helping to prevent vaccine wastage and maintain vaccine potency, VVMs play an essential role in ensuring that communities have access to life-saving immunizations.

Vaccine vial monitors (VVMs) give important information to health workers regarding the temperature of a vaccine. The VVM provides a visual indication of whether the vaccine has been exposed to excessive heat or cold during storage, transport, or handling, which can affect its potency.

Specifically, VVMs provide insights to health workers such as:

1. Vaccine potency status: The color of the VVM indicates whether the vaccine is still potent or has been compromised due to temperature fluctuations. If the VVM shows that the vaccine has been exposed to excessive heat or cold, health workers can discard the vaccine and use an alternative one to ensure the patient receives a potent vaccine.

2. Temperature threshold breaches: The VVM indicates when the temperature of the vaccine has gone above or below the recommended range, indicating potential breaches in temperature control during storage, transport, or handling. This information can help identify areas in the vaccine supply chain that require improvements to maintain the cold chain and prevent temperature excursions.

Overall, VVMs provide critical information to health workers about the temperature history of a vaccine, which helps ensure that potent vaccines are administered to patients and that the vaccine supply chain is properly maintained to preserve vaccine efficacy.

VVM labels can improve efficiency by providing the following:

1. Simple visual indication: The VVM label provides a simple visual indication of whether the vaccine has been exposed to excessive heat or cold. The label changes color based on the temperature history of the vaccine, making it easy for healthcare workers to identify compromised vaccines at a glance.

2. No external equipment needed: VVM labels do not require any external equipment or power supply to function. They are a passive technology that is self-contained within the label itself, making them easy to use and integrate into existing vaccine management systems.

3. Long-lasting: VVM labels have a long shelf life, which means they can potentially remain affixed to vaccine vials for a period of time without losing their effectiveness. This helps reduce the need for frequent label replacements, reducing overall costs and increasing efficiency.

4. Global standardization: VVM labels are a globally standardized technology, which means that they are widely recognized and accepted by healthcare workers, regulatory agencies, and vaccine manufacturers worldwide. This standardization helps ensure that VVM labels are used consistently and correctly, reducing errors and improving efficiency.

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