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TKN (Total Kjeldahl Nitrogen) is the total concentration of organic nitrogen and ammonia. TKN analysis provides the opportunity to quantify the amount of nitrogen contained in organic form. TKN (Total Kjeldahl Nitrogen) this form of nitrogen is defined by the test method used (i. Total Kjeldahl nitrogen or TKN is the sum of nitrogen bound in organic substances, nitrogen in ammonia (NH3-N) and in ammonium (NH4+-N) in the chemical. TKN plus Nitrate Nitrogen (NO3-), Nitrite Nitrogen (NO2-) and Nitrogen gas (N2) can be. Total Nitrogen (TN) should not be confused with TKN (Total Kjeldahl Nitrogen) which is the sum of ammonia-nitrogen plus organically bound nitrogen but does.