What is tmb substrate?
TMB (3,3',5,5'-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP), a common label conjugated to antibodies for applications such as immunohistochemistry, western blotting, and ELISA. 3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising. Upon oxidation, TMB forms a water-soluble blue reaction product that can be measured spectrophotometrically at 605nm. Abcam's TMB ELISA Substrate (High Sensitive) detects horseradish peroxidase ( HRP) activity and contains 3,3',5,5'-tetramethylbenzidine in a mildly acidic. Description: TMB Substrate is ready to use for ELISA detection. TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates.